Anti-Cannabinoid receptor 1/CNR1 Antibody Picoband™

Product Name

Anti-Cannabinoid receptor 1/CNR1 Antibody Picoband™

View all Cannabinoid R1/CB1/CNR1 Antibodies

SKU/Catalog Number

A01291-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cannabinoid receptor 1/CNR1 Antibody Picoband™ catalog # A01291-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Cannabinoid receptor 1/CNR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01291-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human Cannabinoid receptor 1/CNR1 recombinant protein (Position: D27-D466). Human CNR1 shares 96.8% and 97.1% amino acid (aa) sequence identity with mouse and rat CNR1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01291-2 is reactive to CNR1 in Human, Mouse, Rat

Applications

A01291-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

52.858kDa

Background of Cannabinoid R1/CB1/CNR1

The cannabinoid receptor type 1, often abbreviated as CB1, is a G protein-coupled cannabinoid receptor located primarily in the central and peripheral nervous system. This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Cannabinoid Receptor 1/CNR1 using anti-Cannabinoid Receptor 1/CNR1 antibody (A01291-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cannabinoid Receptor 1/CNR1 antigen affinity purified polyclonal antibody (Catalog # A01291-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cannabinoid Receptor 1/CNR1 at approximately 53 kDa. The expected band size for Cannabinoid Receptor 1/CNR1 is at 53 kDa.

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Figure 2. Flow Cytometry analysis of U251 cells using anti-Cannabinoid Receptor 1/CNR1 antibody (A01291-2).
Overlay histogram showing U251 cells stained with A01291-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cannabinoid Receptor 1/CNR1 Antibody (A01291-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CNR1 (Source: Uniprot.org, NCBI)

Alternative Names

CANN6; CANN6CB1R; Cannabinoid R1; cannabinoid receptor 1 (brain); CannabinoidR1; CB1; CB1A; CB1K5; CB1R; CB-R; CB-RCB1cannabinoid receptor 1; central cannabinoid receptor; CNR; CNR1; SKR6R CNR1 CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR cannabinoid receptor 1 cannabinoid receptor 1|cannabinoid receptor 1 (brain)|central cannabinoid receptor

For more info on CNR1, check out the CNR1 Infographic

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In this infographic, you will see the following information for CNR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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